Hsiuping University of Science and Technology Institutional Repository : Item 310993100/1964

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HUSTIR > College of Engineering > Department of Energy and Materials Technology > Monograph > Item 310993100/1964

Please use this identifier to cite or link to this item: http://ir.hust.edu.tw/dspace/handle/310993100/1964

Title:	應用基因重組大腸桿菌生產盤尼西林醯胺酵素
Authors:	王文玉;郭逸萱;詹貴鈞;陳品蓉
Contributors:	化學工程系
Date:	2009-12-30
Issue Date:	2011-01-25T07:50:14Z
Abstract:	利用重組之大腸桿菌發酵可以生產出盤尼西林PGA，主要以大腸桿菌發酵液經過破細胞，上層液即為PGA酵素。本實驗中所使用隻菌種為基因重組大腸桿菌。其帶有 PGL-5 質體可以生產盤尼西林醯胺酵素，為了發酵得到高效率的PGA，利用菌體的生產總量與產物PGA成正比關係，探討各種培養基成分濃度與發酵條件下發酵培養對 PGA 酵素活性之影響，即了解各種培養基成分與培養條件對發酵的影響。由實驗結果得知，當Glucose碳源與Yeast氮源增加時，PGA活性之量也隨之增加；當轉速減少時PGA活性之量也減少；當培養溫度升高時，PGA量則降低。
Appears in Collections:	[Department of Energy and Materials Technology] Monograph

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